High-performance liquid chromatography (HPLC) separates a peptide sample into individual components based on how each compound interacts with the column and mobile phase. In peptide testing, this makes it useful for detecting related substances, truncated sequences, deletion products, isomers, and other impurities that may not be visible in a simple identity check.
How the result is used
The chromatogram shows peaks at different retention times. A major peak often corresponds to the target peptide, while smaller peaks may indicate impurities or degradation products. Labs commonly use area percent to estimate purity, but this value depends on the method, detector settings, and sample characteristics.
- Reversed-phase HPLC is commonly used for peptide purity assessment.
- Method conditions should be reported so results can be interpreted correctly.
- Mass spectrometry is often paired with HPLC to confirm peak identity.
For laboratory procurement, HPLC data should be reviewed alongside the COA and other supporting documentation. Products labeled for research use only are not intended for human consumption.